Zhu L, Maruvada R, Sapirstein A, Malik KU, Peters-Golden M, Kim KS
Arachidonic Acid Metabolism Regulates Escherichia coli Penetration of the Blood-Brain Barrier.
Infect Immun. 2010 Oct;78(10):4302-10
Authors: Zhu L, Maruvada R, Sapirstein A, Malik KU, Peters-Golden M, Kim KS
Escherichia coli K1 meningitis occurs following penetration of the blood-brain barrier, but the underlying mechanisms involved in E. coli penetration of the blood-brain barrier remain incompletely understood. We have previously shown that host cytosolic phospholipase A(2)Î± (cPLA(2)Î±) contributes to E. coli invasion of human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier, but the underlying mechanisms remain unclear. cPLA(2)Î± selectively liberates arachidonic acid from membrane phospholipids. Here, we provide the first direct evidence that host 5-lipoxygenase and lipoxygenase products of arachidonic acid, cysteinyl leukotrienes (LTs), contribute to E. coli K1 invasion of HBMEC and penetration into the brain, and their contributions involve protein kinase C alpha (PKCÎ±). These findings demonstrate that arachidonic acid metabolism regulates E. coli penetration of the blood-brain barrier, and studies are needed to further elucidate the mechanisms involved with metabolic products of arachidonic acid for their contribution to E. coli invasion of the blood-brain barrier.
PMID: 20696828 [PubMed - in process]