Identification of the Biosynthetic Gene Cluster for the Pseudomonas aeruginosa Antimetabolite L-2-Amino-4-Methoxy-trans-3-Butenoic Acid [Physiology and Metabolism]
By Lee, X., Fox, A., Sufrin, J., Henry, H., Majcherczyk, P., Haas, D., Reimmann, C., on July 27th, 2010
See the paper
Lee, X., Fox, A., Sufrin, J., Henry, H., Majcherczyk, P., Haas, D., Reimmann, C.
l-2-Amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction.
