Ujaoney AK, Potnis AA, Kane P, Mukhopadhyaya R, Apte SK
Radiation Desiccation Response Motif-Like Sequences Are Involved in Transcriptional Activation of the Deinococcal ssb Gene by Ionizing Radiation but Not by Desiccation.
J Bacteriol. 2010 Nov;192(21):5637-44
Authors: Ujaoney AK, Potnis AA, Kane P, Mukhopadhyaya R, Apte SK
Single-stranded-DNA binding protein (SSB) levels during poststress recovery of Deinococcus radiodurans were significantly enhanced by (60)Co gamma rays or mitomycin C treatment but not by exposure to UV rays, hydrogen peroxide (H(2)O(2)), or desiccation. Addition of rifampin prior to postirradiation recovery blocked such induction. In silico analysis of the ssb promoter region revealed a 17-bp palindromic radiation/desiccation response motif (RDRM1) at bp -114 to -98 and a somewhat similar sequence (RDRM2) at bp -213 to -197, upstream of the ssb open reading frame. Involvement of these cis elements in radiation-responsive ssb gene expression was assessed by constructing transcriptional fusions of edited versions of the ssb promoter region with a nonspecific acid phosphatase encoding reporter gene, phoN. Recombinant D. radiodurans strains carrying such constructs clearly revealed (i) transcriptional induction of the ssb promoter upon irradiation and mitomycin C treatment but not upon UV or H(2)O(2) treatment and (ii) involvement of both RDRM-like sequences in such activation of SSB expression, in an additive manner.
PMID: 20802034 [PubMed - in process]